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KMID : 1098420110190050373
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Korean Journal of Medicinal Crop Science
2011 Volume.19 No. 5 p.373 ~ p.379
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Suppression Effect of the Inflammatory Response in Macrophages by Paeoniae Radix Rubra Extracts
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Park Jong-Phil
Son Jeong-Hyun
Kim Yong-Min
Jung Joon-Hee
Leem Kang-Hyun
Lee Eun-Yong
Kim E-Hwa
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Abstract
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Paeoniae Radix Rubra is a preparation consisting of desiccated roots of Paeonia lactiflora PALL (belonging to Ranunculaceae). Paeoniae Radix Rubra is used as a medicinal herb in Asian countries to treat many diseases. Ethanol- or water-based extracts of Paeoniae Radix Rubra were prepared and tested on RAW 264.7 cells, a murine macrophage cell line. The expression of some pro-inflammatory proteins, including inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), extracellular signal-regulated kinase 1/2 (ERK1/2) and phosphorylated ERK1/2 was detected by Western blot analyses, while PGE2 expression was quantified by ELISA. Both the water and ethanol extracts of Paeoniae Radix Rubra suppressed LPS-induced nitric oxide (NO) production and exhibited cell toxicity in accordance with increased NO production. Also, both extracts reduced the expression of COX-2 and iNOS, and inhibited phosphorylation of ERK1/2 in LPS-stimulated RAW 264.7 cells. Extracts prepared from Paeoniae Radix Rubra contain anti-inflammatory agents that inhibit the iNOS and MAPK pathways.
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KEYWORD
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Paeoniae Radix Rubra, iNOS, COX-2, ERK1/2, PGE2
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